Antibiofilm Activity of Antarctic Sponge-Associated Bacteria against Pseudomonas aeruginosa and Staphylococcus aureus

Bioprospecting in unusual marine environments provides an innovative approach to search novel biomolecules with antibiofilm activity. Antarctic sponge-associated bacteria belonging Colwellia, Pseudoalteromonas, Shewanella and Winogradskyella genera were evaluated for their ability contrast the biofilm formation by Pseudomonas aeruginosa ATCC 27853 Staphylococcus aureus 29213, as model organisms. All strains able produce at both 4 25 °C, highest production being °C after 24 h. Antibiofilm activity of cell-free supernatants (CFSs) differed among on basis incubation temperature (CFSs4°C CFSs25°C). The major was observed CFSs4°C against S. CFSs25°C P. aeruginosa, without demonstrating a bactericidal effect growth. Furthermore, crude extracts from Colwellia sp. GW185, CAL606, CAL396 also visualized confocal laser scanning microscopic images. Results based surface-coating assay surface tension measurements suggest that CFSs may act biosurfactants inhibiting first adhesion aureus. biopolymers be useful applicative perspectives pharmaceutical environmental purposes.